Thomas Schmidt Lab - Physics of Life Processes


Intrigued by the way cells autonomously regulate their fait we strive to understand and visualize cellular processes as the basis of Cell Signaling. Although extensive knowledge exists about the molecular players, their structure, and their respective interactions, in many cellular signaling pathways the physical and mechanistic properties underlying signaling processes still need to be fully understood. Those cellular processes are ultimately diffusion controlled, hence largely unsynchronized, making them less accessible to typical biochemical bulk methods used so far.

We set out to apply and develop in vivo Single-Molecule Microscopy methods in order to follow the molecular players one-by-one and in real time, thereby obtaining direct information on the physical and mechanistic processes underlying cellular signaling processes. Whereas studies of in vivo systems set the link to biological relevance, complementary in vitro studies do allow us to rigorously test the molecular/mechanistic models which we infer from the in vivo results.


In our efforts we concentrate mainly on the early events in cellular signaling, the moment e.g. a ligand binds to a receptor. Most of such processes take place on the Plasma Membrane of the cell. In relation to signaling processes the two-dimensional environment of the plasma membrane, and its potential nanostructuring, is believed to play a major role in the controlled and robust functioning of signaling pathways. Our emphasis is on unraveling directional sensing as governed by G-Protein Coupled Receptors, and on the initial events that leads to cellular Mechanosensing.



Schmidt News

Our pillars in the news.
Our pillar technology is featured in a technology note of Nature. More info
read more
Publ. 14-06-2017 17:08
AxA postdoctoral fellowship for Stefano Coppola
Stefano Coppola received the prestigious postdoc fellowship from AxA. With this 2-years fellowship he will develop 'Mechanobiology as a novel label-free diagnostic biomarker to predict risks in pancreatic carcinogenesis' in our lab.

Pancreatic ductal adenocarcinoma (PDAC) is the deadliest of all More info

read more
Publ. 10-06-2016 10:46
Rolf's and Mihaela's a-syn paper appeared in PLoS One.
Direct Observation of α-Synuclein Amyloid Aggregates in Endocytic Vesicles of Neuroblastoma Cells. PLoS One (2016) 11, e0153020 More info
read here
Publ. 29-04-2016 19:44
More News

Recent articles

Richter D, Moraga I, Winkelmann H, Birkholz O, Wilmes S, Schulte M, Kraich M, Kenneweg H, Beutel O, Selenschik P, Paterok D, Gavutis M, Schmidt T, Garcia KC, Müller TD, Piehler J (2017) Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments., Nat Commun, 8, 15976.
[Abstract] [DOI] [pdf]

Becchetti A, Crescioli S, Zanieri F, Petroni G, Mercatelli R, Coppola S, Gasparoli L, D'Amico M, Pillozzi S, Crociani O, Stefanini M, Fiore A, Carraresi L, Morello V, Manoli S, Brizzi MF, Ricci D, Rinaldi M, Masi A, Schmidt T, Quercioli F, Defilippi P, Arcangeli A. (2017) The conformational state of hERG1 channels determines integrin association, downstream signaling, and cancer progression., Science Signaling, 10, eaaf3236.
[Abstract] [DOI] [pdf]

Askes SH, Leeuwenburgh VC, Pomp W, Arjmandi-Tash H, Tanase S, Schmidt T, Bonnet S (2017) Water-Dispersible Silica-Coated Upconverting Liposomes: Can a Thin Silica Layer Protect TTA-UC against Oxygen Quenching?, ACS Biomater Sci Eng, 3, 322-334.
[Abstract] [DOI] [pdf]

Askes SH, Pomp W, Hopkins SL, Kros A, Wu S, Schmidt T, Bonnet S (2016) Imaging Upconverting Polymersomes in Cancer Cells: Biocompatible Antioxidants Brighten Triplet-Triplet Annihilation Upconversion., Small, 12, 5579-5590.
[Abstract] [DOI] [pdf]


This email address is being protected from spambots. You need JavaScript enabled to view it.